The Gadsden flag, that includes a rattlesnake and the phrase "Don't Tread on Me

https://kylervbbv051.readspirex.com/posts/buying-guide-what-to-look-for-in-quality-civil-war-flags

The Gadsden flag, presenting a rattlesnake and the word "Don't Tread on Me," was once followed through some Confederate instruments as a image of resistance towards perceived oppression.